Concentration of the CDCP1 protein in human cord plasma may serve as a predictor of hematopoietic stem and progenitor cell content - ScienceDirect

Stem Cell Research

Sofia Frändberg¹ 

关键词: Hematopoietic stem and progenitor cells;    Cord blood;    Proximity ligation assay;    CDCP1;    CB;    cord blood;    HSCT;    hematopoietic stem cell transplantation;    HSPC;    hematopoietic stem and progenitor cell;    CFU;    colony forming unit;    CBBU;    CB buffy coat;    NC;    nucleated cells;    iniNC;    initial CB collection;    iniCD34;    CD34+ cell concentration in initial CB collection;    CBU;    cord blood unit;    CFU-GM;    colony forming unit-granulocyte/monocyte colonies;    PLA;    proximity ligation assay;    NPX;    normalized protein expression;    PCA;    principal component analysis;    FDR;    false discovery rate;   

DOI  :  10.1016/j.scr.2018.03.008

学科分类：生物科学（综合）

来源: Elsevier

【 摘 要 】

Successful hematopoietic stem and progenitor cell (HSPC) transplantation rests upon reliable methods for their enumeration in sources such as cord blood (CB). Methods used today are costly, time consuming and exhaust the limited number of cells needed for transplantation. The aim of this study was to analyze if surplus plasma from CB contains biomarkers that can predict HSPC content in CB. Frozen, surplus plasma from 95 CB units was divided into two groups based on CD34+ cell concentration. Birth weight, gestation age, gender, mode of delivery, collection volume, nucleated cell count and colony forming unit assay results were available. Samples were analyzed with a proximity ligation assay covering 92 different proteins. Two-group t-test with p-values adjusted for false discovery rate (FDR) identified 5 proteins that significantly differed between the two groups. CDCP1 was the most significant (FDR adjusted p-value 0.006). Correlation with CDCP1 concentration was most significant for CD34+ concentration and nucleated cell count. Multivariate analysis showed that CD34 and gender seemed to influence the level of CDCP1. In conclusion, CDCP1 was identified as a potential biomarker of HSPC content in CB. The finding also warrants further investigation for a possible role of CDCP1 in regulating HSPC presence in CB.

【 授权许可】

Unknown