期刊论文详细信息
Developmental biology
The involvement of Eph–Ephrin signaling in tissue separation and convergence during Xenopus gastrulation movements
Edmond ChangkyunPark1 
关键词: EphA4;    Ephrin-A1;    Tissue separation;    CE movements;    Gastrulation;    Brachet';    s cleft;    Xenopus;   
DOI  :  10.1016/j.ydbio.2010.12.012
学科分类:生物科学(综合)
来源: Academic Press
PDF
【 摘 要 】

In Xenopus gastrulation, the involuting mesodermal and non-involuting ectodermal cells remain separated from each other, undergoing convergent extension. Here, we show that Eph–ephrin signaling is crucial for the tissue separation and convergence during gastrulation. The loss of EphA4 function results in aberrant gastrulation movements, which are due to selective inhibition of tissue constriction and separation. At the cellular levels, knockdown of EphA4 impairs polarization and migratory activity of gastrulating cells but not specification of their fates. Importantly, rescue experiments demonstrate that EphA4 controls tissue separation via RhoA GTPase in parallel to Fz7 and PAPC signaling. In addition, we show that EphA4 and its putative ligand, ephrin-A1 are expressed in a complementary manner in the involuting mesodermal and non-involuting ectodermal layers of early gastrulae, respectively. Depletion of ephrin-A1 also abrogates tissue separation behaviors. Therefore, these results suggest that Eph receptor and its ephrin ligand might mediate repulsive interaction for tissue separation and convergence during early Xenopus gastrulation movements.

【 授权许可】

CC BY   

【 预 览 】
附件列表
Files Size Format View
RO201902197336595ZK.pdf 1789KB PDF download
  文献评价指标  
  下载次数:4次 浏览次数:7次