Repeatability and reproducibility of quantitative contrast-enhanced ultrasonography for assessing duodenal perfusion in healthy dogs

The Journal of Veterinary Medical Science

Angkhana DERMLIM¹  Kazuyoshi SASAOKA¹  Masayoshi SHINOHARA¹  Rommaneeya LEELA-ARPORN¹  Khoirun NISA¹  Tatsuyuki OSUGA¹  Tomoya MORITA¹  Sue Yee LIM¹  Mitsuyoshi TAKIGUCHI¹  Nozomu YOKOYAMA¹  Noboru SASAKI¹  Noriyuki NAGATA¹  Hiroshi OHTA¹  Kensuke NAKAMURA²  Keitaro MORISHITA² 

[1] Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan;Veterinary Teaching Hospital, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan

关键词: canine;    duodenum;    quantitative CEUS;    repeatability;   

DOI  :  10.1292/jvms.17-0174

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Contrast-enhanced ultrasonography (CEUS) with microbubbles as a contrast agent allows the visualization and quantification of tissue perfusion. The assessment of canine intestinal perfusion by quantitative CEUS may provide valuable information for diagnosing and monitoring chronic intestinal disorders. This study aimed to assess the repeatability (intraday variability) and reproducibility (interday variability) of quantitative duodenal CEUS in healthy dogs. Six healthy beagles underwent CEUS three times within one day (4-hr intervals) and on two different days (1-week interval). All dogs were sedated with a combination of butorphanol (0.2 mg/kg) and midazolam (0.1 mg/kg) prior to CEUS. The contrast agent (Sonazoid®) was administered using the intravenous bolus method (0.01 ml/kg) for imaging of the duodenum. Time-intensity curves (TIC) were created by drawing multiple regions of interest (ROIs) in the duodenal mucosa, and perfusion parameters, including the time-to-peak (TTP), peak intensity (PI), area under the curve (AUC), and wash-in and wash-out rates (WiR and WoR, respectively), were generated. Intraday and interday coefficients of variation (CVs) for TTP, PI, AUC, WiR and WoR were <25% (range, 2.27–23.41%), which indicated that CEUS was feasible for assessing duodenal perfusion in healthy sedated dogs. A further study of CEUS in dogs with chronic intestinal disorders is necessary to evaluate its clinical applicability.

【 授权许可】

Unknown