Expression of ayu antimicrobial peptide genes after LPSstimulation

The Journal of Veterinary Medical Science

Hiroyuki HORIUCHI¹  Rehab Marray NSRELDEN¹  Shuichi FURUSAWA¹ 

[1] Laboratory of Immunobiology, Graduate School ofBiosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima,Hiroshima 739-8528, Japan

关键词: antimicrobial peptide;    ayu;    innate immunity;    LPS;    mRNA expression;   

DOI  :  10.1292/jvms.16-0609

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Plecoglossus altivelis (ayu) is one of the most important fish speciesin the Japanese islands and in internal fish hatcheries. Living in open aquaticenvironments exposes fish to many pathogens. Therefore, they require rapid and strongimmune defenses. We investigated in vivo the direct association betweenthe ayu innate immune response, represented by the relative transcription of genesencoding the cathelicidin and hepcidin antimicrobial peptides, and lipopolysaccharide(LPS), a conventional pathogen-associated molecular patterns (PAMPs) of Gram-negativebacteria. Different concentrations of LPS (1, 10 and 100 µg/fish) wereinjected intraperitoneally into young (sexually immature) and adult (fully sexuallymature) ayu. The relative expression of the antimicrobial peptide genes was measured 6 hr,24 hr and 1 week after stimulation with LPS. We found a direct association between theexpression of the antimicrobial peptide genes investigated and LPS stimulation. Thisrelationship was time-, dose- and age-dependent. Further research is required to determinethe cell-specific transcriptional regulation and posttranscriptional regulation of theseantimicrobial peptides.

【 授权许可】

Unknown