Chronological differential effects of pro-inflammatory cytokines onRANKL-induced osteoclast differentiation of canine bone marrow-derivedmacrophages

The Journal of Veterinary Medical Science

Eugene C. BWALYA¹  Kenji HOSOYA¹  Masahiro OKUMURA¹  Suranji WIJEKOON¹  Sangho KIM¹  Jing FANG¹ 

[1]Laboratory of Veterinary Surgery, Department ofVeterinary Clinical Sciences, Graduate School of Veterinary Medicine, HokkaidoUniversity, Sapporo 060-0818, Japan

关键词: cytokine;    dog;    osteoclast;    osteoclastogenesis;    rheumatoid arthritis;   

DOI  :  10.1292/jvms.17-0393

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

The aim of this study was to investigate osteoclastogenic properties of inflammatorycytokines at different time-points of osteoclastogenesis. Bone marrow-derived macrophagesfrom five healthy dogs were stimulated with the macrophage colony-stimulating factor,receptor activator of nuclear factor-κB ligand and inflammatory cytokines such asinterleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-17. Osteoclasts (OC) formationand function were enhanced with TNF-α regardless of temporal differences. But in contrast,IL-1β suppressed the osteoclastogenesis at early phase of the process while upregulatingat the late phase. Furthermore, differentiation of OC precursors into OC was suppressed athigh concentrations of IL-17. Collectively, the results revealed that suppressing TNF-αwould be a promising strategy to inhibit inflammation-associated bone destruction indogs.

【 授权许可】

Unknown