Experimental induction of necrotic enteritis in chickens by anetB-positive Japanese isolate of Clostridiumperfringens

The Journal of Veterinary Medical Science

Fumiya KAWAHARA¹  Takayuki SUZUKI¹  Koji UETSUKA¹  Tetsuo NUNOYA¹  Shinya NAGAI¹  Ho TO¹ 

[1] Nippon Institute for Biological Science, 9-2221-1Shinmachi, Ome, Tokyo 198-0024, Japan

关键词: C. perfringens;    fishmeal;    histopathology;    necrotic enteritis;    netB toxin;   

DOI  :  10.1292/jvms.16-0500

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Necrotic enteritis (NE) is one of the most important bacterial diseases in terms ofeconomic losses. Clostridium perfringens necrotic enteritis toxin B,NetB, was recently proposed as a new key virulent factor for the development of NE. Thegoal of this work was to develop a necrotic enteritis model in chickens by using aJapanese isolate of C. perfringens. The Japanese isolate has been foundto contain netB gene, which had the same nucleotide and deduced aminoacid sequences as those of prototype gene characterized in Australian strain EHE-NE18, andalso expressed in vitro a 33-kDa protein identified as NetB toxin bynano-scale liquid chromatographic tandem mass spectrometry. In the challenge experiment,broiler chickens fed a commercial chicken starter diet for 14 days post-hatch were changedto a high protein feed mixed 5050 with fishmeal for 6 days. At day 21 of age, feed waswithheld for 24 hr, and each chicken was orally challenged twice daily with 2ml each of C. perfringens culture (109 to1010 CFU) on 5 consecutive days. The gross necrotic lesions were observed in90 and 12.5% of challenged and control chickens, respectively. To our knowledge, this isthe first study that demonstrated that a netB-positive Japanese isolateof C. perfringens is able to induce the clinical signs and lesionscharacteristic of NE in the experimental model, which may be useful for evaluating thepathogenicity of field isolates, the efficacy of a vaccine or a specific drug againstNE.

【 授权许可】

Unknown