Characterization of Shiga toxin-producing Escherichia coli from feces of sika deer (Cervus nippon) in Japan using PCR binary typing analysis to evaluate their potential human pathogenicity

The Journal of Veterinary Medical Science

Atsushi IGUCHI¹  Tomoko MORITA-ISHIHARA²  Makoto OHNISHI²  Sunao IYODA²  Tomoe ISHIHARA³  Toshiro KUROKI⁴  Eiji YOKOYAMA⁵  Shinichiro HIRAI⁵  Jun TERAJIMA⁶  Shingo SATO⁷  Megumi KAWAMURA⁷  Shinya ODA⁷  Mariko NAGASAKA⁷  Soichi MARUYAMA⁷  Masanari KURANAGA⁷  Hidenori KABEYA⁷ 

[1] Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadainishi, Miyazaki-shi, Miyazaki 889-2192, Japan;Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan;Department of Microbiology, Kanagawa Prefectural Institute of Public Health, 1-3-1 Shimomachiya, Chigasaki-shi, Kanagawa 253-0087, Japan;Department of Planning and Information, Kanagawa Prefectural Institute of Public Health, 1-3-1 Shimomachiya, Chigasaki-shi, Kanagawa 253-0087, Japan;Division of Bacteriology, Chiba Prefectural Institute of Public Health, 666-2 Nitona-cho, Chuo-ku, Chiba-shi, Chiba 260-8715, Japan;Division of Microbiology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan;Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880, Japan

关键词: deer;    Shiga toxin-producing Escherichia coli (STEC);    wild animal;   

DOI  :  10.1292/jvms.16-0568

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

This study examined the potential pathogenicity of Shiga toxin-producing Escherichia coli (STEC) in feces of sika deer by PCR binary typing (P-BIT), using 24 selected STEC genes. A total of 31 STEC strains derived from sika deer in 6 prefectures of Japan were O-serotyped and found to be O93 (n=12), O146 (n=5), O176 (n=3), O130 (n=3), O5 (n=2), O7 (n=1), O96 (n=1), O116 (n=1), O141 (n=1), O157 (n=1) and O-untypable (n=1). Of the 31 STEC strains, 13 carried both stx1 and stx2, 5 carried only stx1, and 13 carried one or two variants of stx2. However, no Stx2 production was observed in 3 strains that carried only stx2 the other 28 strains produced the appropriate Stx. P-BIT analysis showed that the 5 O5 strains from two wild deer formed a cluster with human STEC strains, suggesting that the profiles of the presence of the 24 P-BIT genes in the deer strains were significantly similar to those in human strains. All of the other non-O157 STEC strains in this study were classified with strains from food, domestic animals and humans in another cluster. Good sanitary conditions should be used for deer meat processing to avoid STEC contamination, because STEC is prevalent in deer and deer may be a potential source of STEC causing human infections.

【 授权许可】

Unknown