Development and validation of RP-HPLC method for the estimation of Clonazepam in novel liquisolid compacts, bulk and marketed tablets

Der Pharmacia Lettre

Krishna Sanka¹ 

关键词: Krishna Sanka;    Deepthi Suda;    Sreekar Kethapalli;    Sravanthi Poenti;    Abdul Bari Mohd;    Prakash V Diwan;    RP-HPLC;    Clonazepam;    Liquisolid compacts;    PDA detector;    Purity index;    Dissolution;   

DOI  :  

学科分类：药理学

来源: Scholars Research Library

【 摘 要 】

The present study was designed to develop a simple and rapid reverse phase HPLC method for estimation of clonazepam in novel liquisolid compacts, bulk and marketed formulations. The method was carried out on C18 column using a mixture of 0.1% (v/v) TEA, pH 5.5: Acetonitrile (60:40%v/v) at a flow rate of 1.0 ml/min by using PDA detector at 320nm. Clonazepam showed linearity in the range 31.25 to 500 ng/ml and limits of detection, limit of quantification was 9.2 ng/ml and 27.33 ng/ml, respectively. It can be concluded from the results that the method developed for the estimation of clonazepam in a novel liquisolid compacts, bulk drugs and marketed formulations is a specific, rigid and simple with sufficient sensitivity.

【 授权许可】

CC BY