A novel method of gene transduction to the murine endometrium using in vivo electroporation

The Journal of Veterinary Medical Science

Kanako ENDO¹  Ryosuke KOBAYASHI¹  Eiichi HONDO¹  Yasushige OHMORI¹ 

[1] Laboratory of Animal Morphology, Division of Biofunctional Development, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

关键词: EGFP;    embryo implantation;    in vivo electroporation;    uterus;   

DOI  :  10.1292/jvms.17-0220

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

To investigate the molecular pathways involved in successful embryo implantation in mammals, we developed a novel method for gene transduction into the murine endometrium using in vivo electroporation. Plasmid DNA with an enhanced green fluorescence protein (EGFP) gene was injected into the uterine cavity of non-pregnant female mice, and electrical pulses were subsequently applied to the uterine horn using plate electrodes. EGFP expression was found only in the uterine luminal epithelium (LE), but not in the stroma. EGFP fluorescence in the LE was limited to the site where the positive side of the electrodes was placed during electric stimulation. These results demonstrated that our novel method enabled us to transduce a gene into a desired location of the murine uterus.

【 授权许可】

Unknown