Evaluation of Schistosoma japonicum thioredoxin peroxidase-1 as a potential circulating antigen target for the diagnosis of Asian schistosomiasis

The Journal of Veterinary Medical Science

Elena A. VILLACORTE¹  Lydia R. LEONARDO¹  Pilarita T. RIVERA¹  Masashi KIRINOKI²  Yuichi CHIGUSA²  Yasuyuki GOTO³  Anh TM. DANG⁴  Shin-ichiro KAWAZU⁴  Adrian Miki C. MACALANDA⁴  Luna HIGUCHI⁴  Jose Ma. M. ANGELES⁴  Kharleezelle J. MOENDEG⁴  Xuenan XUAN⁴  Noboru INOUE⁴ 

[1] Department of Parasitology, College of Public Health, University of the Philippines Manila, Philippines;Department of Tropical Medicine and Parasitology, Dokkyo Medical University, Mibu, Tochigi 321-0293, Japan;Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan;National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

关键词: Sandwich ELISA;    Schistosoma japonicum;    schistosomiasis biomarker;    thioredoxin peroxidase-1;   

DOI  :  10.1292/jvms.17-0579

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Asian schistosomiasis caused by Schistosoma japonicum is a serious zoonotic disease endemic in China, the Philippines and parts of Indonesia. Mass drug administration in endemic areas resulted to decline in disease severity and intensity. The low intensity of infection limits the use of current parasitological methods for schistosomiasis diagnosis. Detection of parasite circulating antigens might provide more informative result as it may indicate the true status of infection. In this study, S. japonicum thioredoxin peroxidase-1 (SjTPx-1) a 22 kDa secreted antioxidant enzyme expressed throughout the life stages of the parasite was evaluated for its potential use as a biomarker for schistosomiasis japonica infection. Rabbit polyclonal antibody and mouse monoclonal antibodies (mAbs) were raised against the recombinant SjTPx-1 (rSjTPx-1). The antibodies produced against the recombinant antigen was confirmed to detect the native SjTPx-1 in crude adult worm lysate. Likewise, the specific binding of mAbs to parasite TPx-1 and not to mammalian peroxiredoxin-1 orthologues was also confirmed. The double antibody sandwich ELISA developed in this study was able to detect at least 1 ng/ml of rSjTPx-1. In addition, this method was able to detect the antigen from all serum samples of experimentally infected rabbit and mice. The diagnostic potential of SjTPx-1 in human clinical samples was also evaluated, in which 4 out of 10 stool-confirmed serum samples had detectable levels of the antigen. The results suggest that SjTPx-1 can be a potential biomarker for Asian zoonotic schistosomiasis.

【 授权许可】

Unknown