Molecular characterization and phylogenetic analysis of Explanatumexplanatum in India based on nucleotide sequences of ribosomal ITS2 and themitochondrial gene nad1

The Journal of Veterinary Medical Science

Tadashi ITAGAKI¹  Tambireddy NEERAJA²  Uday K. MOHANTA²  Hiromu SUGIYAMA²  Kei HAYASHI³  T. Shantikumar SINGH³  Yuma OHARI³ 

[1] Department of Aquatic Animal Health Management SriVenkateswara Veterinary University College of Fishery Science, Muthukur 524 344 SPSRNellore, Andhra Pradesh, India;Department of Pathogenetic Veterinary Science, theUnited Graduate School of Veterinary Science, Gifu University, 1–1 Yanagido, Gifu501–1193, Japan;Laboratory of Veterinary Parasitology, Faculty ofAgriculture, Iwate University, 3–18–8 Ueda, Morioka, Iwate 020–8550,Japan

关键词: Explanatum explanatum;    India;    ITS2;    nad1;    phylogeny;   

DOI  :  10.1292/jvms.16-0252

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

The aim of this study was to analyze the phylogenetic relationship betweenExplanatum explanatum populations in India and other countries of theIndian subcontinent. Seventy liver amphistomes collected from four localities in Indiawere identified as E. explanatum based on the nucleotide sequences ofribosomal ITS2. The flukes were then analyzed phylogenetically based on the nucleotidesequence of the mitochondrial gene nad1 in comparison with flukes fromBangladesh and Nepal. In the resulting phylogenetic tree, the nad1haplotypes from India were divided into four clades, and the flukes showing the haplotypesof clades A and C were predominant in India. The haplotypes of the clades A and C havealso been detected in Bangladesh and Nepal, and therefore, it seems they occur commonlythroughout the Indian subcontinent. The results of AMOVA suggested that gene flow waslikely to occur between E. explanatum populations in these countries.These countries are geographically close and have been historically and culturallyconnected to each other, and therefore, the movements of host ruminants among thesecountries might have been involved in the migration of the flukes and their gene flow.

【 授权许可】

Unknown