Effect of a single point mutation on equine herpes virus 9 (EHV-9)neuropathogenicity after intranasal inoculation in a hamster model

The Journal of Veterinary Medical Science

Mohamed NASR¹  Shehata I. ANWAR²  Tokuma YANAI²  Hideto FUKUSHI³  Osama M. ABAS⁴  Hoda A. ABD-ELLATIEFF⁵  Ibrahim SALEH⁶  Asmaa G. SALEH⁶ 

[1] Department of Animal Medicine, Faculty of VeterinaryMedicine, Alexandria University, Alexandria, Egypt;Department of Animal Medicine, Faculty of VeterinaryMedicine, Damanhour University, El-Beheira, Egypt;Department of Pathology and Parasitology, Faculty ofVeterinary Medicine, Damanhour University, El-Beheira, Egypt;Department of Pathology, Faculty of VeterinaryMedicine, Beni-Suef University, Beni-Suef, 62511, Egypt;Laboratory of Veterinary Microbiology, Faculty ofApplied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan;Laboratory of Veterinary Pathology, Faculty of AppliedBiological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan

关键词: 14R;    19R;    EHV-9;    hamster;    neuropathogenicity;   

DOI  :  10.1292/jvms.17-0076

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

This study aimed to investigate the neuropathogenesis of equine herpes virus 9 (EHV-9) bystudying the effects of a single point mutation introduced in two different EHV-9 genes.The two EHV-9 mutants, 14R and 19R, were generated carrying a point mutation in twoseparate EHV-9 genes. These mutants, along with the wild-type EHV-9, were used to infect ahamster model. The EHV-9- and 19R-infected groups showed earlier and more severe clinicalsigns of infection than the 14R-infected group. The white blood cells (WBCs) count wassignificantly increased in both EHV-9- and 19R-infected groups compared to the14R-infected group at the 4th day post infection (DPI). Viremia was also detected earlierin both EHV-9- and 19R-infected groups than 14R-infected group. There were differences inthe anterograde transmission pattern of both EHV-9 and 19R compared to 14R inside thebrain. Serum TNF-α, IL-6 and IFN-γ levels were significantly increased in both EHV-9- and19R-infected groups compared to the 14R-infected group. Histopathological andimmunohistochemical analyses revealed that the mean group scores for the entire brain weresignificantly higher in both EHV-9- and 19R- infected groups than 14R-infected group.Collectively, these results confirm that the gene product of Open Reading Frame 19 (ORF19)plays an important role in EHV-9 neuropathogenicity and that the mutation in ORF19 isresponsible for the attenuation of EHV-9.

【 授权许可】

Unknown