MicroRNA expression profiling in canine prostate cancer

The Journal of Veterinary Medical Science

Kazuhiko OCHIAI¹  Tatsuya HORI²  Masanori KOBAYASHI²  Eiichi KAWAKAMI²  Akiko SAITO²  Makoto BONKOBARA³  Masato KOBAYASHI³  Mami IRIMAJIRI³  Yoshikazu TANAKA⁴  Masaki MICHISHITA⁵  Kimimasa TAKAHASHI⁵  Takeharu KANEDA⁶ 

[1] Department of Basic Science, School of VeterinaryNursing and Technology, Nippon Veterinary and Life Science University, 1-7-1Kyonan-cho, Musashino, Tokyo 180-8602, Japan;Laboratory of Reproduction, Division of TherapeuticScience II, Department of Clinical Veterinary Medicine, School of Veterinary Science,Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo180-8602, Japan;Laboratory of Veterinary Clinical Pathology, Divisionof Therapeutic Science I, Department of Clinical Veterinary Medicine, School ofVeterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho,Musashino, Tokyo 180-8602, Japan;Laboratory of Veterinary Hygiene, Division ofVeterinary Hygiene and Public Health, Department of Preventive Veterinary Medicine,School of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1Kyonan-cho, Musashino, Tokyo 180-8602, Japan;Laboratory of Veterinary Pathology, Division ofPathobiological Analysis, Department of Veterinary Pathobiology, School of VeterinaryScience, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino,Tokyo 180-8602, Japan;Laboratory of Veterinary Pharmacology, Division ofFunctional Morphology, Department of Basic Veterinary Medicine, School of VeterinaryScience, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino,Tokyo 180-8602, Japan

关键词: canine;    expression profiling;    microRNA;    prostate cancer;    real-time PCR;   

DOI  :  10.1292/jvms.16-0279

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Canine prostate cancer (cPCa) is an untreatable malignant neoplasm resulting in localtissue invasion and distant metastasis. MicroRNAs (miRs) are small non-coding RNAs thatfunction as oncogenes or tumor suppressors. The purpose of this study was to characterizethe expression of miRs that are altered in cPCa tissue. The expression levels of 277mature miRs in prostatic tissue (n=5, respectively) were compared between the non-tumorand tumor groups using real-time PCR. Five miRs (miR-18a, 95, 221, 222 and 330) wereup-regulated, but 14 miRs (miR-127, 148a, 205, 299, 329b, 335, 376a, 376c, 379, 380, 381,411, 487b and 495) were down-regulated specifically in cPCa (P<0.05).These miRs have potential use as early diagnosis markers for cPCa and in miR-basedtherapy.

【 授权许可】

Unknown