Comparison of methods for detection of chytrid fungus(Batrachochytrium dendrobatidis) in bullfrog tadpolemouthparts

The Journal of Veterinary Medical Science

Sho KADEKARU¹  Yumi UNE¹ 

[1] Laboratory of Veterinary Pathology, Azabu University,1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan

关键词: Batrachochytrium dendrobatidis;    bullfrog tadpole;    mouthpart;    nested polymerase chain reaction;    swab;   

DOI  :  10.1292/jvms.17-0071

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

We previously reported that the tadpole of bullfrog (Lithobatescatesbeiana) is a useful model for the field surveillance of theBatrachochytrium dendrobatidis (Bd) distribution. In the present study,we compared Bd detection rates in swab-scraped and resected mouthpart samples, usingnested polymerase chain reaction (PCR). The resulting detection rates for swab-scraped andresected specimens were 67 and 65%, respectively, with no significant difference.Furthermore, we performed a histopathological examination for Bd distribution in themouthparts; we found that Bd infection occurred in the tip and basement of the jaw sheathsand tooth rows. We recommend using swab-scraped samples for Bd detection. Moreover,careful attention should be paid to scraping the tip and basement of the jaw sheaths andthe entire oral cavity to reduce the rates of false-negative results on nested PCR of themouthparts of bullfrog tadpoles.

【 授权许可】

Unknown