In situ immobilization of commercial lipase in rigid polyurethane foam and application in the esterification of geraniol and oleic acid

Revista Brasileira de Pesquisa em Alimentos

Elisandra Rigo¹  Jose Wladimir de Oliveira²  Débora de Oliveira²  Sibele Berenice Castellã Pergher³  Ilizandra Aparecida Fernandes⁴  Juliana da Silva Zanatta⁴  Rogério Marcos Dallago⁴  Diane Rigo⁴  Jamile Zeni⁴  Nádia Ligianara Dewes Nyari⁵ 

[1] Departament of Food Engineering, UDESC, Pinhalzinho;Department of Chemical and Food Engineering, UFSC, Florianópolis, 88040-900, SC, Brazil;Department of Chemistry, UFRN, Natal, Brasil;Department of Food Engineering, URI- Av. Sete de Setembro, 1621 - Erechim, 99709-910, RS, Brazil;Universidade Regional Integrada do Alto Uruguai e das Missões – URI – Erechim, RS, Brasil

关键词: Lipase;    immobilization;    Polyurethane;    esterification;    application;   

DOI  :  10.3895/rebrapa.v8n4.4514

学科分类：解剖和生理学

来源: Universidade Tecnica Federal do Parana

【 摘 要 】

The aim of this work was to evaluate the immobilization of Candida antarcticalipase B (CalB) using polyurethane (PU) foam. The stability of the enzymatic derivative against temperature (40, 60 and 80 °C) together with the recycle capacity and storage stability (4 and 25°C) were evaluated. The application of the enzymatic derivative of CalB in esterification reactions was accomplished. Results indicated that the molar ratio between polyol and isocyanate monomers of 5:3 (v/v) was the best one for the immobilization. The immobilization process resulted in a 1363% increase in activity for the enzymatic derivative. The enzymatic derivative obtained kept its initial activity even after 21 h of exposure to high temperatures. The enzymatic activity of the complex was maintained during 30 days at low temperatures, and showed stability during 4 consecutive reuse cycles. The synthesis of geranyl oleate catalyzed by the immobilized derivative presented conversion of 87%. Taking into account the promising results obtained, the low cost of the immobilization support employed and the whole technique developed, this work comprise an innovative contribution.

【 授权许可】

CC BY