Analysis of DNA methylation of potential age-related methylation sites incanine peripheral blood leukocytes

The Journal of Veterinary Medical Science

Yasuyuki MOMOI¹  Genta ITO¹  Kuniko YOSHIMURA¹ 

[1] Laboratory ofVeterinary Diagnostic Imaging, Joint Faculty of Veterinary Medicine, KagoshimaUniversity, 1-21-24 Korimoto, Kagoshima 890-0065, Japan

关键词: age;    dog;    epigenetics;   

DOI  :  10.1292/jvms.16-0341

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Reliable methodology for predicting the age of mature dogs is currently unavailable. Inthis study, amplicon sequencing of 50 blood samples obtained from diseased dogs was usedto measure methylation in seven DNA regions. Significant correlations between methylationlevel and age were identified in four of the seven regions. These four regions were thentested in samples from 31 healthy toy poodles, and correlations were detected in tworegions. The age of another 11 dogs was predicted using data from the diseased dogs andthe healthy poodles. The mean difference between the actual and calculated ages was 34.3and 23.1 months, respectively. Further research is needed to identify additional sites ofage-related methylation and allow accurate age prediction in dogs.

【 授权许可】

Unknown