Blood biochemistry and hematological changes in rats after administration ofa mixture of three anesthetic agents

The Journal of Veterinary Medical Science

Kazuto YAMASHITA¹  Naoto TOYOTA²  Yuichiro OCHIAI²  Toshihiko WATANABE²  Mio HIRAMATSU²  Hiroshi YOKOTA³  Atushi BABA⁴  Hidetomo IWANO⁴ 

[1] Chugai Pharmaceutical Co., Ltd., 1-135 Komakado,Gotemba, Shizuoka 421-8513, Japan;Chugai Research Institute for Medical Science, Inc.,1-135 Komakado, Gotemba, Shizuoka 421-8513, Japan;Department of Small Animal Clinical Sciences, School ofVeterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501,Japan;Laboratory of Veterinary Biochemistry, School ofVeterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501,Japan

关键词: anesthesia;    atipamezole;    butorphanol;    medetomidine;    midazolam;   

DOI  :  10.1292/jvms.17-0497

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Currently, given the concerns regarding animal welfare, it is required that anesthesia oranalgesia be used during surgery in experimental animals. Therefore, it is important tounderstand how anesthesia affects the health conditions of experimental animals. In thisstudy, rat blood biochemistry and hematological changes were examined followingadministration of a mixture of three anesthetic agents—medetomidine, midazolam andbutorphanol (MMB). One of three MMB dose combinations was subcutaneously administered torats. After 1 hr, rats were treated with atipamezole, to reverse the anesthetic effects.Blood biochemistry and hematological parameters were assessed at 1, 4 and 24 hr post-MMBtreatment. We also recorded body weight and food intake at 0, 2, 4, 6 and 24 hr post-MMBadministration. Following MMB administration, transient increases were observed in glucose(GLUC) levels, hematocrit (HCT) values and hemoglobin (HGB) levels, whereas transientdecreases were observed in total protein (TP) content and white blood cell (WBC) counts.Most of these parameters returned to control values 24 hr following MMB administration.Additionally, body weight and food intake decreased in MMB-treated rats. In conclusion,intermediate and high doses of MMB changed some blood biochemistry and hematologicalparameters, body weight and food intake. In contrast, low-dose MMB did not cause theseeffects. Therefore, depending on the experimental design, MMB may influence the results ofstudies that use laboratory animals. Consequently, anesthetic agents used in laboratoryanimals should be chosen based on detailed knowledge of their pharmacological effects.

【 授权许可】

Unknown