Establishment and characterization of a cell line derived fromEptesicus nilssonii

The Journal of Veterinary Medical Science

Haruko OGAWA¹  Kunitoshi IMAI¹  Sachiko MATSUDA²  Masayuki HORIE³  Takumi AKASAKA⁴ 

[1] Diagnostic Center for Animal Health and Food Safety,Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555,Japan;Laboratory of Wildlife Ecology, Obihiro University ofAgriculture and Veterinary Medicine, Obihiro, Hokkaido 080–0555,Japan;Transboundary Animal Diseases Research Center, JointFaculty of Veterinary Medicine, Kagoshima University, Kagoshima 890–0065,Japan;United Graduate School of Veterinary Science, YamaguchiUniversity, Yamaguchi 753–851, Japan

关键词: cell line;    eEBLL-1;    endogenous bornavirus-like element;    Eptesicus nilssonii;    RNA-dependent RNA polymerase;   

DOI  :  10.1292/jvms.16-0274

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Bats of the genus Eptesicus have several non-retroviral RNAvirus-derived sequences in their genomes, among which an endogenous bornavirus-like Lelement, named eEBLL-1, was suggested to encode functional proteins in the hosts. However,the function of eEBLL-1 remains unclear due to a lack of appropriate investigation tools,such as cultured cells expressing eEBLL-1. Here, we established a continuous cell line,named HAMOI-EnK cells, from kidney of Eptesicus nilssonii. HAMOI-EnKcells are robust and could be passaged for at least 10 months. eEBLL-1 in the genomes ofHAMOI-EnK cells retains an intact open reading frame. Additionally, eEBLL-1 is transcribedin the sense-orientation in cells. To our knowledge, this is the first report todemonstrate that eEBLL-1 is transcribed in cultured cells.

【 授权许可】

Unknown