The Journal of Veterinary Medical Science | |
Establishment and characterization of a cell line derived fromEptesicus nilssonii | |
Haruko OGAWA1  Kunitoshi IMAI1  Sachiko MATSUDA2  Masayuki HORIE3  Takumi AKASAKA4  | |
[1] Diagnostic Center for Animal Health and Food Safety,Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080â8555,Japan;Laboratory of Wildlife Ecology, Obihiro University ofAgriculture and Veterinary Medicine, Obihiro, Hokkaido 080â0555,Japan;Transboundary Animal Diseases Research Center, JointFaculty of Veterinary Medicine, Kagoshima University, Kagoshima 890â0065,Japan;United Graduate School of Veterinary Science, YamaguchiUniversity, Yamaguchi 753â851, Japan | |
关键词: cell line; eEBLL-1; endogenous bornavirus-like element; Eptesicus nilssonii; RNA-dependent RNA polymerase; | |
DOI : 10.1292/jvms.16-0274 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
Bats of the genus Eptesicus have several non-retroviral RNAvirus-derived sequences in their genomes, among which an endogenous bornavirus-like Lelement, named eEBLL-1, was suggested to encode functional proteins in the hosts. However,the function of eEBLL-1 remains unclear due to a lack of appropriate investigation tools,such as cultured cells expressing eEBLL-1. Here, we established a continuous cell line,named HAMOI-EnK cells, from kidney of Eptesicus nilssonii. HAMOI-EnKcells are robust and could be passaged for at least 10 months. eEBLL-1 in the genomes ofHAMOI-EnK cells retains an intact open reading frame. Additionally, eEBLL-1 is transcribedin the sense-orientation in cells. To our knowledge, this is the first report todemonstrate that eEBLL-1 is transcribed in cultured cells.
【 授权许可】
Unknown
【 预 览 】
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RO201902191386076ZK.pdf | 561KB | download |