Isolation and characterization of equine dental pulp stem cells derived from Thoroughbred wolf teeth

The Journal of Veterinary Medical Science

Shingo ISHIKAWA¹  Chie HORINOUCHI¹  Kazuhiro MISUMI¹  Daiki MURATA¹  Shota MATSUZAKI¹  Seiji HOBO¹  Yohei IWAMOTO²  Kenji KOROSUE² 

[1] Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan;Miyazaki Yearling Training Farm, Japan Racing Association, 2347 Ohara Hanagashima, Miyazaki 880-0036, Japan

关键词: cell therapy;    dental pulp;    horse;    mesenchymal stem cells;    wolf teeth;   

DOI  :  10.1292/jvms.16-0131

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Mesenchymal stem cells (MSCs) are adult multipotent stem cells that are capable of self-renewal and differentiation into multiple cell lineages. Methods for cell therapy using MSCs have been developed in equine medicine. Recently, human dental pulp stem cells (DPSCs) have drawn much attention owing to their trophic factor producing ability and minimally invasive collection methods. However, there have been no reports on equine dental pulp-derived cells (eDPCs). Therefore, the aim of this study was to isolate and characterize the eDPCs from discarded wolf teeth. Plastic-adherent spindle-shaped cells were isolated from wolf teeth. The doubling time of the isolated eDPCs was approximately 1 day. Differentiation assays using induction medium eDPCs differentiated into osteogenic, chondrogenic and adipogenic lineages. The eDPCs expressed mesenchymal makers (CD11a/18, CD44, CD90 CD105 and MHC class I and II), but did not express hematopoietic markers (CD34 and CD45). Taken together, the results show that eDPCs can be isolated from discarded wolf teeth, and they satisfy the minimal criteria for MSCs. Thus, these eDPCs can be referred to as equine DPSCs (eDPSCs). These eDPSCs may become a new source for cell therapy.

【 授权许可】

Unknown