Increased expression of prion protein gene is accompanied by demethylation of CpG sites in a mouse embryonal carcinoma cell line, P19C6

The Journal of Veterinary Medical Science

Wuyun DALAI¹  Junichi KAWANO¹  Eiko MATSUO¹  Keiichi SAEKI¹  Natsumi TAKEYAMA² 

[1] Laboratory of Microbiology and Immunology, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan;Research Department, Nippon Institute for Biological Science, 9-2221-1 Shinmachi, Ome, Tokyo 198-0024, Japan

关键词: CpG methylation;    epigenetics;    neuronal differentiation;    prion protein gene;   

DOI  :  10.1292/jvms.16-0554

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Elucidation of the processes regulating the prion protein gene (Prnp) is an important key to understanding the development of prion disorders. In this study, we explored the involvement of DNA methylation in Prnp transcriptional regulation during neuronal differentiation of embryonic carcinoma P19C6 cells. When P19C6 cells were differentiated into neuronal cells, the expression of Prnp was markedly increased, while CpG methylation was significantly demethylated at the nucleotide region between −599 and −238 from the transcription start site. In addition, when P19C6 cells were applied in a DNA methyltransferase inhibitor, RG108, Prnp transcripts were also significantly increased in relation to the decreased methylation statuses. These findings helped to elucidate the DNA methylation-mediated regulation of Prnp expression during neuronal differentiation.

【 授权许可】

Unknown