Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia

The Journal of Veterinary Medical Science

Ivo SEDLÁČEK¹  Pavla HOLOCHOVÁ¹  Eva STAŇKOVÁ¹  Pavel Å VEC¹  Juraj BOSÁK²  Gabriela PÁCHNÍKOVÁ²  Iva SLANINOVÁ²  Linda GRILLOVÁ²  David Å MAJS²  Lenka MICENKOVÁ³ 

[1] Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, 625 00, Brno, Czech Republic;Department of Biology, Faculty of Medicine, Masaryk University, 625 00, Brno, Czech Republic;Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, 625 00, Brno, Czech Republic

关键词: Antarctica;    bacteriocins;    cytolethal distending toxin;    Escherichia albertii;   

DOI  :  10.1292/jvms.17-0492

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia. Sequencing of 16S rDNA, automated ribotyping, and rep-PCR were used to identify the four E. albertii isolates. Phylogenetic analyses based on multi-locus sequence typing showed these isolates to be genetically most similar to the members of E. albertii phylogroup G3. These isolates encoded several virulence factors including those, which are characteristic of E. albertii (cytolethal distending toxin and intimin) as well as bacteriocin determinants that typically have a very low prevalence in E. coli strains (D, E7). Moreover, E. albertii protein extracts caused cell cycle arrest in human cell line A375, probably because of cytolethal distending toxin activity.

【 授权许可】

Unknown