Expression profile of matricellular proteins in hypertrophied right ventricleof monocrotaline-induced pulmonary hypertensive rats

The Journal of Veterinary Medical Science

Keisuke IMOTO¹  Hideyuki YAMAWAKI¹  Muneyoshi OKADA¹ 

[1] Laboratory of Veterinary Pharmacology, School ofVeterinary Medicine, Kitasato University, Higashi 23 bancho 35-1, Towada-shi, Aomori034-8628, Japan

关键词: extracellular matrix;    matricellular proteins;    monocrotaline;    pulmonary hypertension;    right ventricular hypertrophy;   

DOI  :  10.1292/jvms.17-0053

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Matricellular proteins, a non-structural extracellular matrix (ECM) component, bind toand modulate various molecules including growth factor, cytokine, protease, other ECMcomponents and cell membrane receptors. While most matricellular proteins are hardlyexpressed in normal adult tissue, they are re-expressed in heart tissue during cardiacdiseases. The present study aimed to clarify the mRNA expression profile of matricellularproteins [secreted protein acidic and rich in cysteine SPARC, hevin, thrombospondin(TSP)-1, -2 and -4, CCN1 and 5, tenascin (Tn) C and N, periostin and osteopontin (OPN)] inhypertrophied right ventricle (RV) of monocrotaline (MCT)-induced pulmonary hypertensiverats. Male Wistar rats were intraperitoneally treated with MCT or saline. Two or threeweeks after MCT treatment, echocardiography was performed, and mRNA expression ofmatricellular proteins was measured by real-time polymerase chain reaction. MCT (2 weeks)induced pulmonary hypertension, RV dysfunction and hypertrophy, which were all worsened 3weeks after MCT treatment. Expression of mRNA for SPARC, hevin, TnC, TSP-1, -2 and -4,CCN1 and 5, periostin and OPN but not TnN was significantly upregulated in RV of MCT (2weeks)-treated rats. Expression of mRNA for TSP-4, CCN1 and 5 and periostin wascontinuously increased in RV of MCT (3 weeks)-treated rats. The present study for thefirst time revealed the mRNA expression profile for matricellular proteins in RV ofMCT-treated rats for 2 or 3 weeks, which will be helpful to clarify the relationship formatricellular proteins and pathogenesis of MCT-induced RV hypertrophy.

【 授权许可】

Unknown