Defect of rabies virus phosphoprotein in its interferon-antagonist activitynegatively affects viral replication in muscle cells

The Journal of Veterinary Medical Science

Kento NAKAGAWA¹  Hiromichi MITAKE²  Kazuma OKADA³  Naoto ITO⁴  Satoko YAMAOKA⁴  Kota OKADERA⁴  Makoto SUGIYAMA⁴ 

[1] Gifu Center for Highly Advanced Integration ofNanosciences and Life Sciences (G-CHAIN), Gifu University, 1-1 Yanagido, Gifu, Gifu501-1193, Japan;Laboratory of Zoonotic Diseases, Faculty of AppliedBiological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193,Japan;Present address: Department of Molecular Medicine, MayoClinic, 200 First Street SW, Rochester, MN 55905, U.S.A.;The United Graduate School of Veterinary Sciences, GifuUniversity, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan

关键词: interferon antagonist;    muscle cells;    phosphoprotein;    rabies virus;   

DOI  :  10.1292/jvms.17-0054

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Attenuated derivative rabies virus Ni-CE replicates in muscle cells less efficiently thandoes the parental pathogenic strain Nishigahara. To examine the mechanism underlying theless efficient replication of Ni-CE, we compared the activities of Ni-CE and Nishigaharaphosphoproteins, viral interferon (IFN) antagonists, to suppress IFN-β promoter activityin muscle cells and we demonstrated a defect of Ni-CE phosphoprotein in this ability.Treatment with an IFN-β-neutralizing antibody improved the replication efficiency of Ni-CEin muscle cells, indicating that produced IFN inhibits Ni-CE replication. The resultsindicate the importance of IFN antagonism of rabies virus phosphoprotein for viralreplication in muscle cells.

【 授权许可】

Unknown