Mass cytometry deep phenotyping of human mononuclear phagocytes and myeloid-derived suppressor cells from human blood and bone marrow

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

Lhomme, Faustine¹  Diggins, Kirsten E.¹  Irish, Jonathan M.⁶  Le Gallou, Simon⁷  Johnson, Douglas B.⁷  Ferrell, P. Brent⁹  Greenplate, Allison R.^1,10 

[1] Mixte de Recherche U1236, UniversitéRennes, Etablissement Francais du Sang Bretagne, Equipe Labelliséand;..Department of Cancer Biology and Vanderbilt‐..INSERM, UnitéCHU de Rennes, Pole de Biologie, Rennes, France;Department of Cancer Biology and Vanderbilt‐Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA;Department of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA;Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA;e Ligue Contre le Cancer, Rennes, France

关键词: CyTOF;    MDSCs;    macrophages;    monocyte;   

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

The monocyte phagocyte system (MPS) includes numerous monocyte, macrophage, and dendritic cell (DC) populations that are heterogeneous, both phenotypically and functionally. In this study, we sought to characterize those diverse MPS phenotypes with mass cytometry (CyTOF). To identify a deep phenotype of monocytes, macrophages, and DCs, a panel was designed to measure 38 identity, activation, and polarization markers, including CD14, CD16, HLA-DR, CD163, CD206, CD33, CD36, CD32, CD64, CD13, CD11b, CD11c, CD86, and CD274. MPS diversity was characterized for 1) circulating monocytes from healthy donors, 2) monocyte-derived macrophages further polarized in vitro (i.e., M-CSF, GM-CSF, IL-4, IL-10, IFN-γ, or LPS long-term stimulations), 3) monocyte-derived DCs, and 4) myeloid-derived suppressor cells (MDSCs), generated in vitro from bone marrow and/or peripheral blood. Known monocyte subsets were detected in peripheral blood to validate the panel and analysis pipeline. Then, using various culture conditions and stimuli before CyTOF analysis, we constructed a multidimensional framework for the MPS compartment, which was registered against historical M1 or M2 macrophages, monocyte subsets, and DCs. Notably, MDSCs generated in vitro from bone marrow expressed more S100A9 than when generated from peripheral blood. Finally, to test the approach in vivo, peripheral blood from patients with melanoma (n = 5) was characterized and observed to be enriched for MDSCs with a phenotype of CD14⁺HLA-DR^lowS100A9^high (3% of PBMCs in healthy donors, 15.5% in patients with melanoma, P < 0.02). In summary, mass cytometry comprehensively characterized phenotypes of human monocyte, MDSC, macrophage, and DC subpopulations in both in vitro models and patients.

【 授权许可】

CC BY