Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
The effect of plasma auto-IgGs on CD4+ T cell apoptosis and recovery in HIV-infected patients under antiretroviral therapy | |
Li, Zhen1  Jiang, Wei1  Ma, Lei1  Ogunrinde, Elizabeth1  Heath, Sonya1  Qin, Zhiqiang1  Wan, Zhuang4  Ou, Tongwen5  Huang, Lei9  Martin, Lisa9  Zhou, Zejun9  Zhang, Tao9  Liao, Guoyang1,10  Wu, Hao1,12  Zhang, Jiafeng1,15  | |
[1] Peking Union Medical College, Kuming, China;and;..The First Affiliated Hospital of Hu‐Beijing You’Biologicals Department, Institute of Medical Biology, Chinese Academy of Medical Sciences &Capital Medical University Affiliated XuanWu Hospital, Xicheng District, Beijing, China;Department of Genetics, Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA;Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina, USA;Division of Infectious Diseases, Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA;Division of Infectious Diseases, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA;Nan University of Chinese Medicine, Changsha, China;The People's Liberation Army No. 302 Hospital, Treatment and Research Center for Infectious Diseases, Beijing, China;Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China;an Hospital, Capital Medical University, Fengtai District, Beijing, China | |
关键词: disease; autoantibody; HIV infection; ART; | |
学科分类:生理学 | |
来源: Federation of American Societies for Experimental Biology | |
【 摘 要 】
Although effective antiretroviral therapy (ART) suppresses HIV viral replication, prevents AIDS-related complications, and prolongs life, a proportion of patients fails to restore the patients’ CD4+ T cell number to the level of healthy individuals. Increased mortality and morbidity have been observed in these patients. In the current study, we have investigated the role of auto-IgGs in CD4+ T cell apoptosis and recovery in a cross-sectional study. All HIV+ subjects were on viral-suppressive ART treatment with a different degree of CD4+ T cell reconstitution. Total auto-IgG binding on CD4+ T cell surfaces and its associated apoptosis and CD4+ T cell recovery were analyzed by flow cytometry ex vivo. Total IgGs from plasma were tested for their binding capacities to CD4+ T cell surfaces and their mediation to CD4+ T cell death through NK cell cytotoxicity in vitro. HIV+ subjects had increased surface binding of auto-IgGs on CD4+ T cells compared with healthy controls, and IgG binding was associated with elevated CD4+ T cell apoptosis in HIV+ subjects but not in healthy controls. Plasma IgGs from HIV+ subjects bound to CD4+ T cells and induced cell apoptosis through NK cytotoxicity in vitro. Soluble CD4 (sCD4) preincubation prevented NK cell-mediated CD4+ T cell death. Our results suggest that plasma autoantibodies may play a role in some HIV+ patients with poor CD4+ T cell recovery under viral-suppressive ART.
【 授权许可】
CC BY
【 预 览 】
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RO201902189706398ZK.pdf | 80KB | download |