The effect of plasma auto-IgGs on CD4+ T cell apoptosis and recovery in HIV-infected patients under antiretroviral therapy

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

Li, Zhen¹  Jiang, Wei¹  Ma, Lei¹  Ogunrinde, Elizabeth¹  Heath, Sonya¹  Qin, Zhiqiang¹  Wan, Zhuang⁴  Ou, Tongwen⁵  Huang, Lei⁹  Martin, Lisa⁹  Zhou, Zejun⁹  Zhang, Tao⁹  Liao, Guoyang^1,10  Wu, Hao^1,12  Zhang, Jiafeng^1,15 

[1] Peking Union Medical College, Kuming, China;and;..The First Affiliated Hospital of Hu‐Beijing You’Biologicals Department, Institute of Medical Biology, Chinese Academy of Medical Sciences &Capital Medical University Affiliated XuanWu Hospital, Xicheng District, Beijing, China;Department of Genetics, Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA;Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina, USA;Division of Infectious Diseases, Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA;Division of Infectious Diseases, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA;Nan University of Chinese Medicine, Changsha, China;The People's Liberation Army No. 302 Hospital, Treatment and Research Center for Infectious Diseases, Beijing, China;Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China;an Hospital, Capital Medical University, Fengtai District, Beijing, China

关键词: disease;    autoantibody;    HIV infection;    ART;   

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Although effective antiretroviral therapy (ART) suppresses HIV viral replication, prevents AIDS-related complications, and prolongs life, a proportion of patients fails to restore the patients’ CD4⁺ T cell number to the level of healthy individuals. Increased mortality and morbidity have been observed in these patients. In the current study, we have investigated the role of auto-IgGs in CD4⁺ T cell apoptosis and recovery in a cross-sectional study. All HIV⁺ subjects were on viral-suppressive ART treatment with a different degree of CD4⁺ T cell reconstitution. Total auto-IgG binding on CD4⁺ T cell surfaces and its associated apoptosis and CD4⁺ T cell recovery were analyzed by flow cytometry ex vivo. Total IgGs from plasma were tested for their binding capacities to CD4⁺ T cell surfaces and their mediation to CD4⁺ T cell death through NK cell cytotoxicity in vitro. HIV⁺ subjects had increased surface binding of auto-IgGs on CD4⁺ T cells compared with healthy controls, and IgG binding was associated with elevated CD4⁺ T cell apoptosis in HIV⁺ subjects but not in healthy controls. Plasma IgGs from HIV⁺ subjects bound to CD4⁺ T cells and induced cell apoptosis through NK cytotoxicity in vitro. Soluble CD4 (sCD4) preincubation prevented NK cell-mediated CD4⁺ T cell death. Our results suggest that plasma autoantibodies may play a role in some HIV⁺ patients with poor CD4⁺ T cell recovery under viral-suppressive ART.

【 授权许可】

CC BY