Hepatitis C Virus (HCV) RNA Quantification and Typing for the Identification of HCV Genotypes in clinical isolates

International Journal of Biomedical and Advance Research

Narotam Sharma ; Meenakshi Bahuguna ; Sneha Rawat ; Neelam Panwar ; Priyanka Chauhan ; Rakhi Kaushik ; Satish Chandra Nautiyal ; R K Singh

关键词: s HCV genotypes, Nested PCR, Viral load, Real time PCR, Amplicon, Quantitation Standard;   

学科分类：药学、药理学、毒理学（综合）

来源: Scholar Science Journals

【 摘 要 】

The frequency of infection with the major genotypes of hepatitis C virus (HCV) was investigated in the current study. 30 serum specimens were quantified for HCV RNA viral load and their titer values were observed with the usage of Real Time PCR in-between 1.05x101-9.58x107IU/ml. HCV genotype 3a was found in 17(56.6%) cases with highest prevalence. Genotypes 1a, 1b, 2a, 2b ,3b ,4, and 5a was found 2(6.6%),15(50%),5(16.6%),15(50%),2(6.6%),2(6.6%),2(6.6%) respectively. The findings also included that HCV viral RNA titre was elevated in HCV genotype 3a, assuming that HCV genotype 3a is the most replicated virus and need to be monitored thoroughly during diagnosis. Although type 1a, 1b, 2a, 2b, 3b, 4,5a were also present but in lower proportion.

【 授权许可】

CC BY