In vitro Research of the Concentration Dependence of Effect of Adrenaline on Platelets Aggregation

Current Health Sciences Journal

E. Mati ; Alice Piperea-Sianu ; I. SARBU ; A. CROITORU ; STILIYANA BORISOVA

关键词: Adrenaline;    Platelet Aggregation;    DLVO;   

学科分类：医学（综合）

来源: Universitatea de Medicina si Farmacie din Craiova / University of Medicine and Pharmacy

【 摘 要 】

Purpose. Paper intended to present experimental evidences that adrenaline has a direct effect of inducing platelets aggregation in the concentration range 1-8M. Material/ Methods. Platelet rich plasma from patients of Colentina Clinical Hospital, following an informed consent. The platelet rich plasma (PRP) was prepared by centrifuging the anticoagulated sample at 200 G for 10 minutes. Aggregation was evaluated by optical aggregometry, classical method of Born, using Helena PACKS-4 Aggregometer. Results. The curves transmission light-time followed the structure: a lag-time, a first phase aggregation, more or less linear, defined by a lope 1? a second wave of aggregation defined by lope 2?and a aturation?phase. Slope 1 increases with the concentration of adrenaline. The second slopes of the aggregation curves, maximum aggregation and areas under curves depended linear on adrenaline concentration. Conclusions. Adrenaline, in concentrations in the 1-8M, induce aggregation of human platelets from platelet rich plasma. Linear regression models for slope and area were practically identical suggesting a rather unique than biphasic mechanism of action of adrenaline during the time course of aggregation.

【 授权许可】

Unknown