Investigation of

Pakistan Veterinary Journal

Mehmet Demirci¹  Ferruh Tombul²  Akın Yiğin³ 

[1] 1University of Istanbul, Cerrahpaşa Medical Faculty, Medical Microbiology, 34089, Istanbul–Turkey;2Republic of Turkey, Ministry of Food, Agriculture and Livestock, Istanbul Food Control Biogenetic laboratory, Istanbul-Turkey;3University of Harran, Faculty of Veterinary Medicine, Department of Genetic, 63000, Şanlıurfa – Turkey

关键词: Direct detection;    Enterotoxin genes;    Food;    Real-time PCR;    Staphylococcus aureus;   

学科分类：兽医学

来源: University of Agriculture * Faculty of Veterinary Science

【 摘 要 】

The aim of this study was to investigate the Staphylococcal enterotoxin (SE) and directly detect the five classical SEA, SEB, SEC, SED and SEE gene in Staphylococcus aureus strains from different food samples by real-time PCR. We studied totally 3650 different food samples such as milk and dairy products, meat and meat products, poultry and eggs, canned food, coffee, cocoa and derived products, honey, confectionery and bakery, ready-to-eat foods, beverages in food control laboratory. We found that a total of 36 (0.98%) S. aureus strains were isolated and only 14 (0.38%) out of 36 S. aureus strains were found to be enterotoxingenic. Milk samples were found to be most contaminant among the products. The most prevalent SE types were SEA 7 (19.4%). Commercial EIA kit results were used to compare with the real-time PCR results. SE results were found to be same with these two methods. This study showed that prevalence and type of the staphylococcal enterotoxin may vary from food to food. It is important to know this data to prevent outbreaks. Additionally, automated DNA isolation and real-time PCR methods can be performed to direct enterotoxin gene detection rapidly and reliably. This technique can be also used for food safety and clinical diagnosis applications.

【 授权许可】

CC BY-NC