Extracción y morfología del embrión de Bursera bipinnata (Burseraceae)

Acta Botanica Mexicana

Judith Márquez-Guzmán¹  Valeria Flores-Enríquez¹  María del Coro Arizmendi²  María Felix Ramos-Ordoñez² 

[1] Universidad Nacional Autónoma de México, Facultad de Ciencias, Laboratorio de Desarrollo en Plantas, Avenida Universidad 3000, Coyoacán, Ciudad de México, México;Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Iztacala, UBIPRO, Laboratorio de Ecología, Avenida de los Barrios núm. 1, Los Reyes Ixtacala, 54090 Tlalnepantla, Estado de México, México

关键词: Burseraceae;    cotiledón;    embriogénesis;    pireno;    tecnica de extracción;    semilla;   

DOI  :  10.21829/abm117.2016.1165

学科分类：植物学

来源: Acta Botanica Mexicana

【 摘 要 】

Background and Aims:The understanding of the reproductive biology of plants, from the formation of the seed to its establishment, allows the development of different conservation strategies. In the genusBursera , whose main center of diversification is Mexico, the diaspore is a pyrene formed by a strongly lignified endocarp enclosing the seed. The anatomical characteristics of the pyrene and the parthenocarpy are the main factors that have hindered the study of embryogenesis and the development of propagation techniques. In this paper we describe the morphology of theBursera bipinnataembryo, extracted by a technique that does not alter the structure or the chemical composition of tissues. Methods:During the beginning of the dispersal stage 2014, we collected unripe fruits and pyrenes with exposed pseudaril. Unripe fruits were fixed in FAA. A low speed micromotor with handpiece was used. A conical burr was used in unripe fruits to scabble the fruit valves. To open the pyrenes a diamond disc was used. After opening the endocarp, embryos were removed and fixed. Key results:The pattern of development coincides with other model species of angiosperms. In immature fruits globular, heart-shaped, torpedo and cotyledonary stages were observed, the latter in early stages. During the torpedo stage, cotyledons branch into three lobes and are spliced, as they grow, they are folded upon themselves, and cover the embryonic axis; however, the radicular meristem is always exposed. Conclusions:The extraction technique is rapid, inexpensive and non-destructive to tissues, having a potential use in the detailed analysis of embryogenesis in the genusBursera , tissue culture andex situconservation.

【 授权许可】

CC BY-SA