CD11d β2 integrin expression on human NK, B, and γδ T cells

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

Postovit, Lynne-Marie¹  Dekaban, Gregory A.³  Barreira, Christy R.⁴ 

[1] ..Department of Microbiology and Immunology, The University of Western Ontario, London, Ontario, Canada;Department of Oncology, University of Alberta, Edmonton, Alberta, Canada;Molecular Medicine, Robarts Research Institute, The University of Western Ontario, London, Ontario, Canada

关键词: acute spinal cord injury;    innate immunity;    inflammation;    gamma delta T cells;    IL‐;    2;   

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

The CD11d integrin is expressed on the cell surface of leukocytes that belong to the myeloid lineage, but its expression on lymphocytes remains unexplored. To test the hypothesis that CD11d is expressed on lymphocyte subsets, we employed a multicolor flow cytometry panel to identify CD11d expression on B, NK, CD4⁺ and CD8⁺ αβ T cells (αβTc), and γδ T cells (γδTc) in human PBMC samples. CD11d was highly expressed on NK cells, B cells, and γδTc, but not αβTc. CD11d expression was higher on freshly isolated γδTc compared with αβTc from healthy donors, yet both inter- and intradonor variability was evident. Over time in primary culture, we consistently observed higher CD11d levels on γδTc compared with αβTc from the same donor. Furthermore, CD11d expression on γδTc increased over time and correlated with levels of IL-2 supplementation. Of interest, a greater percentage of Vδ1 γδTc expressed CD11d than did Vδ2 γδTc, which suggested differential roles for this integrin that may segregate with γδTc subsets. These results expand the potential for CD11d to regulate lymphocyte migration and tissue retention, and illuminate the possibility of a previously unconsidered role for CD11d in leukocyte biology and disease.

【 授权许可】

CC BY