Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1

IUCrJ

Schlichting, I.¹  Weierstall, U.¹  Kirian, R.A.¹  Doak, R.B.¹  Fromme, P.¹  Hunter, M.S.¹  Boutet, S.²  Mittman, M.²  Yu, H.²  Spence, J.C.H.²  Dörner, K.²  Shoeman, R.L.³  Lee, H.-H.³  Liu, H.³  Doran, J.D.³  Seibert, M.M.³  Fromme, R.³  Aquila, A.³  Chapman, H.N.³  Basu, S.³  Stellato, F.⁴  Mor, T.S.⁴  Matoba, N.⁴  Nass, K.⁴  Schmidt, K.E.⁴  Wang, D.⁴  Lawrence, R.M.⁴  James, D.⁵  Zatsepin, N.A.⁵  Yoon, C.⁶  Hogue, B.G.⁶  Grotjohann, I.⁷  Cherni, I.⁷  Barty, A.⁷  Deb, A.⁷  Williams, G.J.⁸  Kupitz, C.⁸ 

[1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany;Center for Infectious Diseases and Vaccinology, Biodesign Institute, Arizona State University, PO Box 874501, Tempe, AZ 85287-5401, USA;Department of Chemistry and Biochemistry, Arizona State University, PO Box 871604, Tempe, AZ 85287-1604, USA;Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287-1504, USA;Linac Coherent Light Source, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA;Max-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany;School of Life Sciences, Arizona State University, PO Box 874501, Tempe, AZ 85287-4501, USA;University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany

关键词: X-RAY CRYSTALLOGRAPHY;    FEMTOSECOND NANOCRYSTALLOGRAPHY;    HIV-1;    GP41;    MEMBRANE-PROXIMAL REGION;    CHOLERA TOXIN B SUBUNIT;    CRYSTALLIZATION;    FREE-ELECTRON LASERS;   

DOI  :  10.1107/S2052252514014900

学科分类：数学（综合）

来源: International Union of Crystallography

【 授权许可】

CC BY