HPLC analysis and blood-brain penetration of 20-hydroxyecdysone diacetonide

Acta Chromatographicae

Huba Kalász¹  Attila Hunyadi²  Kornélia Tekes³  Rafael Dolesal⁴  Gellért Karvaly⁵ 

[1] 1Department of Pharmacology and Pharmacotherapy, Semmelweis University, 1089 Budapest, Nagyvárad tér 4, Hungary;2Institute of Pharmacognosy, University of Szeged, 6720 Szeged, Eötvös u. 6, Hungary;3Department of Pharmacodynamics, Semmelweis University, 1089 Budapest, Nagyvárad tér 4, Hungary;4University Hospital Hradec Kralove, 500 05 Hradec Kralove, Sokolska 581, Czech Republic;5Department of Laboratory Medicine, Semmelweis University, 1089 Budapest, Nagyvárad tér 4, Hungary

关键词: HPLC;    ecdysteroid;    20-hydroxyecdysone 2,3,20,22-diacetonide;    blood-brain barrier penetration;    distribution;   

DOI  :  10.1556/1326.2017.29.3.07

学科分类：化学（综合）

来源: Akademiai Kiado Rt.

【 摘 要 】

Blood-brain penetration of 20-hydroxyecdysone 2,3;20,22-diacetonide (20DA) has been scouted using chromatographic methods. In vivo experiments were performed by treating male Wistar rats intraperitoneally (i.p.) with a dose of 50 mg kg−1 20DA. Control experiment was done by using 20-hydroxyecdysone (20E). Definite brain penetration of 20DA was found by using high-performance liquid chromatography (HPLC), while 20E does not show this type of distribution.

【 授权许可】

Unknown