Down-regulation of CD73 on B cells of patients with viremic HIV correlates with B cell activation and disease progression

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

Schmidt, Reinhold E¹  Wroblewski, Raluca¹  th, Ilona¹  Lunzen, Jan¹  Haag, Friedrich³  Geyer, Matthias⁷  Bockhorn, Maximilian⁷  Eberhard, Johanna M.⁷  Tó⁷  Beisel, Claudia⁹  Schulze zur Wiesch, Julian⁹  Ackermann, Christin⁹  Dierks, Patrick⁹  Scherg, Felix⁹ 

[1] ..Center for Immunology and Rheumatology, Hannover Medical School, Hannover, Germany;..German Center for Infection Research, Hamburg, Hannover, Germany;Center of Advanced European Studies and Research, Bonn, Germany;Department of General Surgery, University Medical Center Hamburg‐Department of Immunology, University Medical Center Hamburg‐Eppendorf, Eppendorf, Germany;German Center for Infection Research, Hamburg, Hannover, Germany;I. Department of Medicine, Section of Infectious Diseases, University Medical Center Hamburg‐Viiv Healthcare, London, United Kingdom

关键词: CD39;    5′    ‐;    ectonucleotidase;    elite controller;    ADO;    Ig class switch;    CD39;   

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Recently, alterations of the T cell expression of the ectonucleotidases, CD39 and CD73, during HIV infection have been described. Here, peripheral (n = 70) and lymph nodal B cells (n = 10) of patients with HIV at different stages of disease as well as uninfected individuals were analyzed via multicolor flow cytometry with regard to expression of CD39 and CD73 and differentiation, proliferation, and exhaustion status. Patients with chronic, untreated HIV showed a significantly decreased frequency of CD73-expressing B cells (P < 0.001) compared with healthy controls. Decreased frequencies of CD39⁺CD73⁺ B cells in patients with HIV correlated with low CD4⁺ counts (P < 0.0256) as well as increased proliferation and exhaustion status as determined by Ki-67 and programmed death-1 expression. Down-regulation of CD73 was observed in naive and memory B cells as determined by CD27 and CD21. Neither HIV elite controller patients nor antiretroviral therapy–treated patients had significantly lower CD39 and CD73 expression on B cells compared with healthy controls. Of importance, low CD73⁺ expression on B cells was associated with modulated in vitro B cell function. Further in vivo studies are warranted to evaluate the in vivo role of phenotypic loss of CD73 in B cell dysregulation in HIV.

【 授权许可】

CC BY