CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

Nardone, Glenn¹  Kirkpatrick, Charles H.¹  Break, Timothy J.¹  Reckhow, Jensen D.¹  Datta, Sandip K.¹  Myers, Timothy G.¹  Lionakis, Michail S.⁴  Gardina, Paul J.⁴  Holland, Steven M.⁴  Saleem, Danial⁵  Zhao, Ming⁵  Olano, Lisa R.⁵ 

[1] and;Genomic Technologies Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA;Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA;Research Technologies Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA;University of Colorado Health Science Center, Denver, Colorado, USA

关键词: transfer factor;    cellular immunity;    Tc17;   

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Cellular lysates from PPD⁺ donors have been reported to transfer tuberculin reactivity to naïve recipients, but not diphtheria reactivity, and vice versa. A historically controversial topic, the terms “transfer factor” and “DLE” were used to characterize the reactivity-transferring properties of lysates. Intrigued by these reported phenomena, we found that the cellular extract derived from antigen-specific memory CD8⁺ T cells induces IL-6 from antigen-matched APCs. This ultimately elicits IL-17 from bystander memory CD8⁺ T cells. We have identified that dialyzable peptide sequences, S100a9, and the TCR β chain from CD8⁺ T cells contribute to the molecular nature of this activity. We further show that extracts from antigen-targeted T cells enhance immunity to Staphylococcus aureus and Candida albicans. These effects are sensitive to immunization protocols and extraction methodology in ways that may explain past discrepancies in the reproducibility of passive cellular immunity.

【 授权许可】

CC BY