Exosomes from eosinophils autoregulate and promote eosinophil functions

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

Manuel¹  Quirce, Santiago¹  oz, José³  n⁵  Sastre, Joaquí⁶  Gonzá⁸  Sastre, Beatriz⁸  lez-Guerra, André⁹  Mazzeo, Carla⁹  Pozo, Victoria⁹  Barranco, Pilar^1,12  Rodrigo-Muñ^1,12  s^1,14  Ferná^1,14  ndez-Nieto, Mar^1,15  Izquierdo, Manuel^1,15 

[1] and;..CIBER de Enfermedades Respiratorias (CIBERES), Madrid, Spain;..Department of Allergy, Hospital La Paz‐..Department of Allergy, IIS‐CIBER de Enfermedades Respiratorias (CIBERES), Madrid, Spain;Department of Biochemistry, Instituto de Investigaciones BioméDepartment of Immunology, IIS‐FundacióInstitute for Health Research (IdiPAZ), Madrid, Spain;UAM, Facultad de Medicina, Universidad Autóaz, Madrid, Spain;dicas Alberto Sols, CSIC‐n Jiménez Dínoma de Madrid, Madrid, Spain

关键词: eosinophil migration;    eosinophil adhesion;    oxidative stress;   

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Eosinophils are able to secrete exosomes that have an undefined role in asthma pathogenesis. We hypothesized that exosomes released by eosinophils autoregulate and promote eosinophil function. Eosinophils of patients with asthma (n = 58) and healthy volunteers (n = 16) were purified from peripheral blood, and exosomes were isolated and quantified from eosinophils of the asthmatic and healthy populations. Apoptosis, adhesion, adhesion molecules expression, and migration assays were performed with eosinophils in the presence or absence of exosomes from healthy and asthmatic individuals. Reactive oxygen species (ROS) were evaluated by flow cytometry with an intracellular fluorescent probe and nitric oxide (NO) and a colorimetric kit. In addition, exosomal proteins were analyzed by mass spectrometry. Eosinophil-derived exosomes induced an increase in NO and ROS production on eosinophils. Moreover, exosomes could act as a chemotactic factor on eosinophils, and they produced an increase in cell adhesion, giving rise to a specific augmentation of adhesion molecules, such as ICAM-1 and integrin α2. Protein content between exosomes from healthy and asthmatic individuals seems to be similar in both groups. In conclusion, we found that exosomes from the eosinophils of patients with asthma could modify several specific eosinophil functions related to asthma pathogenesis and that they could contribute fundamentally to the development and maintenance of asthma.

【 授权许可】

CC BY