Combinative effect of Salvia sclarea L., Artemisia annua and Dracocephalum heterophyllum B. essential oils against Salmonella enterica in raw chicken

Dynamic Chiropractic

关键词: Antibacterial activities;    essential oils;    food-borne pathogens;    fractional inhibitory concentration;    minimum inhibitory concentration;    Salmonella enterica.;   

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Antibacterial properties of essential oils (EOs) extracted fromSalvia sclarea, Artemisia annuaandDracocephalum heterophyllumagainst17 food borne pathogens was studied. EOs of the three plants showed a broad spectrum of antimicrobial activity with different degrees of inhibition against the tested Gram-positive and Gram-negative bacteria. EOs ofSalviaandDracocephalumdepicted bactericidal mode of action while that ofArtemisiainhibited the bacteria with bacteriostatic mode.Salmonella entericaMTCC 733 was the most sensitive strain toSalvia, ArtemisiaandDracocephalumEOs with minimum inhibitory concentration (MIC) values of 2000, 2000 and 8000 µg/ml, respectively. The antimicrobial activity of EOs individually and in combinations based on their respective MIC values againstS. entericawas tested in raw chicken. Treatment of food sample with 20 times MIC value ofS. sclarea, A. annuaandD. heterophyllumEOs individually caused reduction of bacterial load to 3.36, 3.64 and 4.22 log cfu/g after 180 min. In contrast the bacterial cell loads reduced to an undetectable level by the combinative effect (Salvia+ DracocephalumandSalvia + Artemisia)of EOs at MIC value after 120 and 180 min, respectively.This study suggests that combinations of EOs could minimize application concentrations in real food system.

【 授权许可】

Unknown