期刊论文详细信息
Munis Entomology Zoology
Partial characterization of digestive α-amylase in cotton bollworm, Helicoverpa armigera H�bner (Lepidoptera: Noctuidae)
R. F. Pour Abad1 
关键词: Helicoverpa armigera;    α;    -amylase;    developmental stages;   
DOI  :  
学科分类:昆虫学
来源: Munis Research Group
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【 摘 要 】

 In this study α-amylase activity of different developmental stages of cotton bollworm were studied. Insects reared on chickpea based artificial diet in controlled condition. Alpha-Amylase activity was determined using 4, 6-ethylidene (G7)-p-nitrophenyl-(G1)-α-D-maltoheptaoside as substrate. The most enzyme activity was observed in 6th larval stage. Male and female adults and pupas showed a minor α-amylase activity. Lumen content of 6th larval midgut showed significantly more enzyme activity in comparison with midgut tissues during circadian cycle. Starvation period non-significantly affected α-amylase activity in whole body and gut extract of 6th larval stage alimentary canel assays. Enzyme activity during embryogenesis and pupa developing period were studied and results revealed that with aging of eggs amylase activity increased but just before hatching, it started to decrease. In pupal growth period, amylase activity showed a decline manner to last period of developing days. Also the results showed that, the optimal pH for α-amylase activity in 6th larval stage of cotton bollworm was alkaline (pH 12)

【 授权许可】

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