Antiprotozoal activity and cytotoxicity of metabolites from leaves of Teclea trichocarpa

Dynamic Chiropractic

E. S. K. Mwangi¹  J. M. Keriko²  A. K. Machocho³ 

[1] Department of Chemistry, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200 Nairobi, Kenya.;Department of Chemistry, Kenyatta University, P. O. Box 43844-00100 Nairobi, Kenya.;Department of Physical Science (Chemistry), Masinde Muliro University of Science and Technology, P. O. Box 190 - 50100 Kakamega, Kenya.

关键词: Cytotoxicity;    Teclea trichocarpa;    protozoa;    alkaloids.;   

DOI  :  10.5897/JMPR10.188

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Chromatographic separation of the leaves ofTeclea trichocarpa(Engl.) Engl. (Rutaceae) used traditionally by Akamba tribe in Kenya yielded three acridone alkaloids, a furoquinoline alkaloid and two triterpenoids. The total extract (methanol) of the leaves of this plant and the isolated compounds were screened forin vitrofor cytotoxicity and against parasitic protozoa,Plasmodium falciparum,Trypanosoma brucei rhodesiense,Trypanosoma cruziandLeishmania donovani. Among the compoundsa-amyrin had the best anti-plasmodial activity (IC50= 0.96mg/ml), normelicopicine and skimmianine had the best anti-trypanosomal activity againstT.b.rhodesiense(IC50= 5.24mg/ml) andT. cruzi(IC50= 14.50mg/ml), respectively. Normelicopicine also exhibited best anti-leishmanial activity (IC50= 1.08mg/ml). Arborinine exhibited moderate cytotoxicity (IC50= 12.2mg/ml) against L-6 cells. The compounds with low anti-protozoal and high cytotoxicity IC50values are potential source of template drug against parasitic protozoa.

【 授权许可】

Unknown