Inhibition of Telomerase Activity by Cucurbitacin I in Colon Cancer Cell Line, LS174T

Proceedings

Tosun, Emir¹ 

关键词: cucurbitacin I;    telomerase activity;    human telomerase reverse transcriptase messenger ribonucleic acid;    LS174T cell line;   

DOI  :  10.3390/proceedings1101017

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Telomerase activity is dependent on the expression level of human telomerase reverse transcriptase (hTERT) in cancer cells. hTERT messenger ribonucleic acid (mRNA) is expressed in more than 85% of cancer cells, but not in normal human somatic cells. Therefore, the proliferation of human cancer cells can be arrested by telomerase inhibition. We aimed to investigate the effect of cucurbitacin I on the inhibition of hTERT mRNA expression in LS174T colorectal carcinoma cells. After treating the LS174T cells with various concentrations (2.5–125 ng/mL) of cucurbitacin I for 24 h, total RNA was extracted and cDNA was synthesized. Real-time PCR was used for quantitative assessment of hTERT mRNA gene expression versus control group. The level of hTERT mRNA was calculated via normalized to β-actin mRNA level which uniformly expressed housekeeping gene, within each sample. The results of our investigation suggested that cucurbitacin I significantly inhibited telomerase activity and showed a considerable decrease in mRNA expression of hTERT in the treated cells in comparison with the control cells in a concentration-dependent manner. Preliminary study shows that cucurbitacin I is effective for the inhibition of telomerase activity in colorectal cancer.

【 授权许可】

CC BY