Triptolide inhibits cytokine-induced activation of retro-ocular fibroblasts from patients with Graves ophthalmopathy

Dynamic Chiropractic

Lv Jiu-sheng¹  YAN Shu-xun²  Lin Yan-jie²  Zhang bin³  Sun Ya-ru⁴  Feng Zhi-hai⁴  WANG Ying⁴ 

[1] Department of Chinese Medicine Pharmacology, Henan College of Traditional Chinese Medicine, Zhengzhou City 450008, Henan Province, People’Department of Endocrinology and Metabolism, The First Affiliated Hospital, Henan College of Traditional Chinese Medicine, Zhengzhou City 450008, Henan province, People’Department of Geriatrics, The First Affiliated Hospital, Zhengzhou University, Zhengzhou City 450000, Henan province, People’s Republic of China.

关键词: Triptolide;    graves’    ophthalmopathy;    retro-ocular;    fibroblasts;    cytokine.;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

To explore the effects of Triptolide which has immunosuppressive and anti-inflammatory properties on the expression of human leucocyte antigen-DR (HLA-DR), intercellular adhesion molecule-1 (ICAM-1), CD40 and Fas enduced by cytokines and the synthesis of hyaluronic acid (HA) on cultured retro-ocular fibroblasts (RFs) from patients with Graves' ophthalmopathy. Cultured RFs were incubated with IL-1 (100U/mL) or TNF-α (100U/mL) in the presence of Triptolide. Flow cytometry was used to investigate the amount of HLA-DR, ICAM-1, CD40 and Fas. HA synthesis was measured by radioimmunoassay. At base conditions, the percentage of positive cells of HLA-DR, ICAM-1 and CD40 on RFs were 6.70 ± 3.06%, 14.89 ± 11.67% and5.29 ± 3.02%, and the synthesis of HA was337.8 ± 42.7 ng/ml. IL-1 or TNF-α significantly enhanced the amount of HLA-DR, ICAM-1 and CD40 and HA synthesis. Triptolide 0.01µg/L had little effect on cytokine- induced HLA-DR, ICAM-1 and CD40 amounts, as well as HA synthesis. When the concentration ranged from 0.1to 10µg/L, Triptolide inhibited cytokine- induced RFs activation in a dose-dependent manner. Triptolide itself also induced Fas expression on RFs in a dose-dependent manner. Triptolide could inhibit cytokines-induced activation of RFs derived from patients with Graves' ophthalmopathy.

【 授权许可】

Unknown