Genomic DNA extraction from seed induced callus and explants in Salvia L. species for utilization in secondary metabolite production

Dynamic Chiropractic

关键词: Callus;    genomic DNA extraction;    in vitroculture;    SalviaL. species;   

DOI  :  10.5897/JMPR11.1382

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Salvia, the largest genus of Lamiaceae, includes about 900 species, widespread throughout the world. This genus is represented, in Turkish flora, by 88 species and 93 taxa, 45 of which are endemic. Some members of this genus are of economic importance since they have been used as flavoring agents in perfumery and cosmetics. Despite the medicinal potential of plants in Turkey being considerable, knowledge of this area and studies on these crops is scarce. IncludingSalviaL. species, in some medicinal plants low callus production, especially leave-based ones, is the main problem inin vitroculture, due to phenolic compounds. Callus induction, therefore, from plant's non-specialized tissues, for example, seeds, is inevitable. The six species ofSalviaL. growing naturally in Anatolia (Salvia aetopisL., Salvia cadmica Boiss., Salvia candidissimaVahlssp. occidentalisHedge,Salvia cryptanthaMontbretetAucher ex Bentham (endemic to Turkey),Salvia tomentosaMillerandSalvia verticillataL. ssp. verticillataL.) were selected. Callus of the seeds of the aforementioned species was initiated on MS basal media supplemented with combinations of indole-butyric acid (IBA) and picloram. Varied mass callus induction was observed in all media but,Salvia tomentosaMiller was found to be most efficient species in callusing. Moreover, applying the Lefort method, the extracted genomic DNA for all the samples was pure and ideal.

【 授权许可】

Unknown