In vitro and in vivo antioxidant properties of Tunisian carob (Ceratonia siliqua L.)

Dynamic Chiropractic

Kais Rtibi¹  Latifa Chehimi³  Mohamed Amri⁵  Hichem Sebai⁶  Abdelaziz Souli⁷  #⁸  Mohsen Sakly⁹  Jamel El-Benna^1,12 

[1] des Sciences de Bizerte, 7021 Zarzouna, Tunisia.;des Sciences de Tunis. Campus Universitaire El Manar II-2092 Tunis, Tunisia.;FacultéLaboratoire de Neurophysiologie Fonctionnelle et Pathologies, DéLaboratoire de Nutrition et Physiologie Animale - Institut SupéLaboratoire de Physiologie Intée, gréja, Avenue Habib Bourguiba - B. P. 382 - 9000 Béja, Tunisia.;partement des Sciences Biologiques, Facultérieur de Biotechnologie de Bé

关键词: Carob;    phenolic compounds;    antioxidant properties;    rat.;   

DOI  :  10.5897/JMPR12.915

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The present study aims at the quantification of phenolic compounds of Tunisian carob (Ceratonia siliquaL.) pods and the study of theirin vitroandin vivoantioxidant activities in different extraction solvents. In this respect, we used 50 rats divided into five groups: Control and Carob-treated groups (100, 200, 600 and 1000 mg/kg,b.w.). Our results showed that the carob polar extracts are richer in total polyphenols, total flavonoids and condensed tannins than the nonpolar extracts with quantitative variation of phenolic compounds between seeds and pulp.In vitro, the determination of antioxidant capacity by 2,2'-azino-bis[3-ethylbenzthiazoline-6-sulphonic acid] (ABTS) method showed that carob extracts present high antioxidant potency in a solvent-compartment-dependent way compared to Trolox, an antioxidant reference molecule.In vivo, the subacute treatment within 7 days with aqueous extract of carob pods (AECP) decreased significantly and doses dependently the cerebral and myocardial lipid peroxidation as well as the level of hydrogen peroxide (H2O2) in kidney, liver and brain but not in heart. These data suggest that carob fruit presents anin vitroanin vivoantioxidant effects and might be proposed as a food additive to protect against oxidative stress damage.

【 授权许可】

Unknown