Evaluation of antioxidant, oxidative DNA damage protective and antimicrobial activities of Foeniculum vulgare plant

Dynamic Chiropractic

关键词: Total phenolic content;    antioxidant;    reducing power;    herbal drug.;   

DOI  :  10.5897/JMPR2013.5120

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The potential health benefits ofFoeniculum vulgare(FV), commonly known as fennel,is related to the treatment of cardiovascular disease, digestive disorders, carminative, diuretic, etc. Thepresent study was carried out to evaluateF. vulgareplant as new source of natural bioactive molecules having antioxidant potential andDNA damage protection activity caused by reactive oxygen species (ROS).Among ethanol, methanol, hexane, chloroform, butanol, acetone and aqueous extracts, ethanol extract of matureF. vulgareplant leaf showed the highest total phenolic content (TPC; 44.11 mg of gallic acid equivalent (GAE)/g) and the lowest inhibitory concentration (IC50) for free radical scavenging activity (FRSA) (1.95 mg/ml), superoxide anion radical scavenging activity (SARSA) (7.02 mg/ml) and hydroxyl radical scavenging activity (23.53 mg/ml). Methanol and hexane extracts had the lowest IC50for lipid peroxidation (LPO) (9.68 mg/ml) and ferric thiocyanate assay (FTC) (0.92 mg/ml), respectively. Ethanol extracts showed the highest reducing power (RP, 1.22 ascorbic acid equivalent (ASE)/ml) proving itself to be the best solvent. In further studies, the antioxidant activity at early and mature stages of plant was compared. Ethanol extract of matureF. vulgareleaf had the highest TPC and maximum percentage inhibition for FRSA, SARSA, RP, LPO, FTC, hydroxyl radical scavenging activity and had maximum protection against DNA damage in pBR322 plasmid. Results showed thatF. vulgareis a rich source of many biomolecules having antioxidant activities and showed potential utility of mature plant for use in herbal drug system or as nutritional supplement.

【 授权许可】

Unknown