Induced-differentiation of two flavones and two flavonols on a human esophageal squamous cell carcinoma cell line (KYSE-510)

Dynamic Chiropractic

Qiang Zhang¹  Zhu-Jun Wang² 

[1] Department of Food Science, Northeast Agricultural University, Harbin 150030, P. R. China.;Key Laboratory of Dairy Science of Ministry of Education, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China.

关键词: Flavones;    flavonols;    differentiation;    esophageal cancer.;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The effects of two flavones (baicalein, chrysin) and two flavonols (galangin, kaempferol) on induction of differentiation in a human esophageal squamous cell carcinoma (KYSE-510) cell line were investigated in this study. Four compounds were found to be able to inhibit proliferation of KYSE-510 cells in a dose- and time-dependent manner. The inhibitory potency of them on the growth of KYSE-510 cells was in the order ofgalangin>chrysin>baicalein>kaempferol. The KYSE-510 cells treated with these compounds for 24 h showed differentiation characteristics including the differentiation-specific morphological changes, the augmentation of differentiation markers and the inhibition of human telomerase reverse transcriptase. The potency of these compounds on induction of differentiation was similar to that of them on growth inhibition. To elucidate the possible mechanisms by which these compounds modulate proliferation and differentiation in KYSE-510 cells, the alterations of threegenes(p21waf1, cyclin B1 and D1) related to proliferation and differentiation were investigated by real-time RT-PCR and Western blot. The results showed that the up-regulation of p21waf1and down-regulation of cyclin B1 and D1 at the mRNA and protein levels in KYSE-510 cells treated with four compounds were observed, which implied that p21waf1, cyclin B1 and D1 might be targetgenesof thesecompoundsin inducing differentiation.

【 授权许可】

Unknown