The Effect of Tocopherol-α on the Cell Viability in Caco-2 Cell Line

Proceedings

Gök, Ayşenur¹ 

关键词: Antiproliferative effect;    Caco-2 cell line;    tocopherol-α.;   

DOI  :  10.3390/proceedings1101062

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Tocopherols, the major forms of vitamin E, are a family of fat-soluble phenolic compounds. Each tocopherol contains a chromanol ring system and a phytyl chain containing 16 carbons. Tocopherols, as effective antioxidants, have been proposed to protect against carcinogenesis. Colon carsinoma cell line were treated with tocopherol-α (0, 3.12, 6.25, 12.5, 25, 50, 100, 200 uM). Cell viability and migration were examined. Cell viability was determined MTT assay and cell migration was determined by wound healing assay. Caco-2 cells were treated with for 24 h, 48 h and 72 h incubation. There is a significant decrease was observed at 50, 100 and 200 µM for 48 h and 72 h incubation on cell viability in the MTT assay. Wound healing method observed decrease on migration at 12, 5, 25 and 50 µM in 24 h. These results suggest that tocopherol-α have promising antiproliferative effect on cell viability for research on cancer.

【 授权许可】

CC BY