Relationship between mycelium morphology and extracellular polysaccharide production of medicinal mushroom Ganoderma lucidum in submerged culture

Dynamic Chiropractic

Lin Peng¹  Zhongyang Ding²  Qiong Wang³ 

[1]National Engineering Laboratory for Cereal fermentation Technology, Jiangnan University, Wuxi 214122, China.

[2]The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.

[3]The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.

关键词: Medicinal mushroom;    Ganoderma lucidum;    morphology;    extracellular polysaccharides;    submerged fermentation.;   

DOI  :  10.5897/JMPR11.1523

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The medicinal mushroomGanoderma lucidumis a source of polysaccharides used in disease treatment. The objective of this study was to investigate the relationships betweenG. lucidummycelium morphology and the production of extracellular polysaccharides (EPS) in submerged fermentation. Mycelium pellets were classified according to diameter as either S, M or L pellets. M pellets were the main source of EPS. Changes in mycelium morphology and EPS production were obtained when cells were grown in different culture media; medium that contained a high concentration of glucose and low peptone enhanced the formation of M pellets. The proportion of M pellets in the culture was influenced greatly by KH2PO4concentration and increase was accompanied by enhanced EPS production. Dry cell weight was affected mostly by the percentage of total M and L pellets present, while S pellet numbers had little effect. The production of EPS and the accumulation of mycelia were influenced mainly byG. lucidumpellet morphology. This study suggests that in large scale fermentation, high yields of EPS would be obtained by the control of mushroom morphology.

【 授权许可】

Unknown