Antibacterial, antioxidant and phytochemical investigation of Thuja orientalis leaves

Dynamic Chiropractic

关键词: Thuja orientalis;    2;    2-diphenyl-1-picrylhydrazyl(DPPH);    extracts;    fractions;    minimum inhibitory concentrations (MICs).;   

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

In the present study, leaves ofThuja orientaliswere powdered and extracted by soxhlet extractor in two solvent systems that is, (E1) ethyl acetate: chloroform: ethanol (40: 30: 30) and (E2) methanol: distilled water (70:30). This study conferred the screening of phytochemical constituents, antioxidant activity and antibacterial activity of crude E1and E2extract and its fractions. Antioxidant activity was carried out by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The results indicate that E2extract (70% methanolic extract) had the highest antioxidant effect (85.25% inhibition) at 100 µg/ml concentration and the crude extracts (E1and E2extract) showed significant (P ≤ 0.05) inhibitory activity against both gram positive and gram negative organisms. It was active againstStaphylococcus aureus, Bacillus subtilis, Escherichia coli, andAgrobacterium tumefaiens.The minimum inhibitory concentrations (MICs) of E1extract ranged from 0.40 to 0.85 mg/ml and E2extract 0.55 to 1.15 mg/ml. The highest antibacterial potentiality was exhibited by E2extract. The fractions also exhibited antimicrobial activity against all the selected microorganisms. The study revealed thatT. orientalisis a promising phytomedicine for antioxidant and antibacterial activity.

【 授权许可】

Unknown