The Dendritic Cell Populations of Mouse Lymph Nodes

The Journal of Immunology: Official Journal of the American Association of Immunologists

Jason Waithman¹  Sem Saeland¹  Ken Shortman¹  David Vremec¹  Christophe Benoist¹  Emanuela Handman⁵  Kim Burnham⁶  Stuart Williams⁶  Sandrine Henri⁶  Arun Kamath⁶ 

[1]Joslin Diabetes Center, Boston, MA 02215

[2]Laboratory for Immunological Research, Schering-Plough, Dardilly France

[3]Oklahoma State University, Stillwater, OK 74078

[4]Scripps Clinic and Research Foundation, La Jolla, CA 92037

[5]Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia

DOI  :  10.4049/jimmunol.167.2.741

学科分类：生物科学（综合）

来源: American Association of Immunologists

【 摘 要 】

The dendritic cells (DC) of mouse lymph nodes (LN) were isolated, analyzed for surface markers, and compared with those of spleen. Low to moderate staining of LN DC for CD4 and low staining for CD8 was shown to be attributable to pickup of these markers from T cells. Excluding this artifact, five LN DC subsets could be delineated. They included the three populations found in spleen (CD4+8−DEC-205−, CD4−8−DEC-205−, CD4−8+DEC-205+), although the CD4-expressing DC were of low incidence. LN DC included two additional populations, characterized by relatively low expression of CD8 but moderate or high expression of DEC-205. Both appeared among the DC migrating out of skin into LN, but only one was restricted to skin-draining LN and was identified as the mature form of epidermal Langerhans cells (LC). The putative LC-derived DC displayed the following properties: large size; high levels of class II MHC, which persisted to some extent even in CIITA null mice; expression of very high levels of DEC-205 and of CD40; expression of many myeloid surface markers; and no expression of CD4 and only low to moderate expression of CD8. The putative LC-derived DC among skin emigrants and in LN also showed strong intracellular staining of langerin.

【 授权许可】

CC BY