Increased Plasmodium falciparum Parasitemia in Non-splenectomized Saimiri sciureus Monkeys Treated with Clodronate Liposomes

Frontiers in Cellular and Infection Microbiology

da Silva, Igor J.¹  Andrade, Má¹  Daniel-Ribeiro, Clá⁴  Cunha, Janaiara A.⁴  Pratt-Riccio, Lilian R.⁴  Druilhe, Pierre⁷  rcia C. R.⁷  Pelajo-Machado, Marcelo⁹  Bianco-Junior, Cesare⁹  Riccio, Evelyn K. P.^1,11  Carvalho, Leonardo J. M.^1,11  udio Tadeu^1,11 

[1] ã, Paris, France;Instituto de CiêLaboratóVac4All Initiative, Pepiniència e Tecnologia em Biomodelos, Fiocruz, Rio de Janeiro, Brazil;o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil;re Paris Biotech Santéria, Instituto Oswaldo Cruz, Fundaçrio de Patologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil;rio de Pesquisa em Malá

关键词: Malaria;    Plasmodium falciparum;    Saimiri sciureus;    Experimental models;    Clodronate liposomes;    Macrophages;    Spleen;    Liver;   

DOI  :  10.3389/fcimb.2017.00408

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

A major constraint in the study of Plasmodium falciparum malaria, including vaccine development, lies on the parasite’s strict human host specificity and therefore the shortage of animal experimental models able to harbor human plasmodia. The best experimental models are neo-tropical primates of the genus Saimiri and Aotus, but they require splenectomy to reduce innate defences for achieving high and consistent parasitemias, an important limitation. Clodronate-liposomes (CL) have been successfully used to deplete monocytes/macrophages in several experimental models. We investigated whether a reduction in the numbers of phagocytic cells by CL would improve the development of P. falciparum parasitemia in non-splenectomized Saimiri sciureus monkeys. Depletion of S. sciureus splenocytes after in vitro incubation with CL was quantified using anti-CD14 antibodies and flow cytometry. Non-infected and P. falciparum-infected S. sciureus were injected intravenously twice a week with either CL at a low rate of either 0.5 or 1 mL (5mg/mL) or phosphate buffered saline (PBS). Animals were monitored during infection and treated with mefloquine. After treatment and euthanasia, spleen and liver were collected for histological analysis. In vitro CL depleted S. sciureus splenic monocyte/macrophage population in a dose- and time-dependent manner. In vivo, half of P. falciparum-infected S. sciureus treated with CL 0.5 mL, and two-thirds of those treated with CL 1 mL developed high parasitemias requiring mefloquine treatment, whereas all control animals were able to self-control parasitemia without the need for antimalarial treatment. CL-treated infected S. sciureus showed a marked decrease in the degree of splenomegaly despite higher parasitemias, compared to PBS-treated animals. Histological evidence of partial monocyte/macrophage depletion, decreased hemozoin phagocytosis and decreased iron recycling was observed in both the spleen and liver of CL-treated infected S. sciureus. CL is capable of promoting higher parasitemia in P. falciparum-infected S. sciureus, associated with evidence of partial macrophage depletion in the spleen and liver. Macrophage depletion by CL is therefore a practical and viable alternative to surgical splenectomy in this experimental model.

【 授权许可】

CC BY