First report of nematodes Parasitylenchus bifurcatus Poinar & Steenberg, 2012 parasitizing multicolored Asian lady beetle Harmonia axyridis (Pallas, 1773) in Slovenia

Acta Agriculturae Slovenica

Barbara Gerič Stare¹  Gregor UREK¹  Saša ŠIRCA¹ 

[1] Kmetijski inštitut Slovenije

关键词: parasitic nematodes;    ladybirds beetles;    Parasitylenchus bifurcatus;    Harmonia axyridis;    harlequin ladybird;    biological control;   

DOI  :  10.14720/aas.2017.109.2.28

学科分类：农业科学（综合）

来源: Univerza v Ljubljani * Biotehniska Fakulteta / University of Ljubljana * Biotechnical Faculty

【 摘 要 】

In years 2015 and 2016 around 200 specimens of ladybird speciesHarmonia axyridiswere collected in Slovenia. Ladybirds were dissected and checked under stereomicroscope for presence of nematodes. Nematodes were discovered in samples of ladybirdsH. axyridiscollected at Brdo pri Lukovici and Trboje. The incidence of parasitized ladybirds was around 30 %. Nematodes were analysed morphometrically and the characteristic features ofParasitylenchus bifurcatusnematodes were observed: straight stylet lacking basal thickenings, a bursa and a forked tail tip in the vermiform females and juvenile males. The identity was confirmed with 18S rDNA region sequence (acc. no. LT629306 and LT629307) which showed high similarity (>99.9 % nucleotide identity) to theP. bifurcatussequences in the public domain. This is a first report ofP. bifurcatusnematode species found parasitizing ladybirdHarmonia axyridisin Slovenia. The species compromises fecundity of its host and has therefore a potential to be used as a biological control agent to control high abundance of invasiveH. axyridisladybirds .

【 授权许可】

CC BY-NC-ND